Studies in Spermatogenesis Volume I Part 3

22. First maturation spindle in metaphase, chromosomes in single and double diamond shapes.

23. Chromosome in single diamond or tetrad form, as they usually come into the spindle.

24. Double diamond-form a.s.sumed before metakinesis.

25. The 26 chromosomes of an early metaphase.

26. First maturation spindle in metakinesis.

27. Equatorial plate of first maturation spindle in metakinesis.

28. Another spindle, showing three granules which are probably remains of nucleoli.

29. Anaphase of first maturation mitosis, one centrosome divided.

30. Late anaphase.

31-32. Telophase, exceptional cases of division of the cell.

33-36. Partial rest stage between first and second maturation divisions, two nucleoli present. Chromosomes in fig. 36 in form of double diamonds ready for metakinesis.

37-38. Second maturation spindle in metaphase.

39. Equatorial plate of second maturation spindle, 26 chromosomes.

40. Same in anaphase.

41. Four spermatid nuclei in one cell, each nucleus containing one nucleolus.

42. A later stage, showing elongation of nuclei, centrosome and sphere at posterior end.

43-45. Later stages in the development of the spermatozoa, nucleolus grows gradually smaller.

[Ill.u.s.tration: STEVENS. PLATE I.

N. M. S. del.

TERMOPSIS ANGUSTICOLLIS.]

PLATE II.

_Stenopelmatus._

FIGS. 46-47. Nuclei of spermatogonia, showing 2 and 3 nucleoli (_n_).

48-49. Prophase of spermatogonial mitosis, showing two exceptionally large chromosomes of equal length.

50. Equatorial plate of spermatogonial mitosis, 46 chromosomes.

51-54. Spermatocytes in spireme stage, nucleus containing a nucleolus (_n_), and a chromatin element (_x_), which is attached to one end of spireme and gradually increases in size during growth stage of spermatocytes.

55. Spireme longitudinally split and showing the beginning of cross formation.

56. Spireme segmented, tetrads forming.

57. One split segment and a part of another connected by bands of linin.

58. More open cross and diamond forms; element _x_ conspicuous.

59-60. More contracted cross and diamond-shaped tetrads; linin bands shown in 60, where element _x_ is also present.

61. Different forms a.s.sumed by element _x_ during tetrad stage (figs.

56-60).

62-63. Diamond-shaped and contracted cross-shaped tetrads from metaphase of first maturation mitosis, showing linin connections.

64. Diamond-shaped tetrad with spindle-fibers attached; _a-a_, probably halves of one univalent chromosome; _b-b_, halves of the other.

65. Dyad from anaphase of first maturation mitosis.

66-67. Metaphase of first maturation spindle, showing element _x_ in different positions.

68. Late anaphase of same.

69-70. Equatorial plate of first maturation spindle, 23 chromosomes and element _x_ below, in fig. 69.

71. Chromatin ma.s.sed at poles of spindle; element _x_ isolated at one pole.

72-73. Two resting spermatocytes of the second order, one containing element _x_, the other not.

74-76. Successive stages of breaking down of element _x_.

77. Prophase of second division; dyads evident, but no sign of _x_ in this or following stages.

78. Second spermatocyte division--metakinesis.

79. Same; late anaphase.

[Ill.u.s.tration: STEVENS. PLATE II.

N. M. S. del.

STENOPELMATUS.]

PLATE III.