The Microscope Part 1

The Microscope.

by Andrew Ross.

THE MICROSCOPE.

Microscope, the name of an instrument for enabling the eye to see distinctly objects which are placed at a very short distance from it, or to see magnified images of small objects, and therefore to see smaller objects than would otherwise be visible. The name is derived from the two Greek words, expressing this property, MIKROS, _small_, and SKOPEO, _to see_.

So little is known of the early history of the microscope, and so certain is it that the magnifying power of lenses must have been discovered as soon as lenses were made, that there is no reason for hazarding any doubtful speculations on the question of discovery. We shall proceed therefore at once to describe the simplest forms of microscopes, to explain their later and more important improvements, and finally to exhibit the instrument in its present perfect state.

In doing this we shall a.s.sume that the reader is familiar with the information contained in the articles "Light," "Lens," "Achromatic,"

"Aberration," and the other sub-divisions of the science of Optics, which are treated of in this work.

The use of the term _magnifying_ has led many into a misconception of the nature of the effect produced by convex lenses. It is not always understood that the so-called magnifying power of a lens applied to the eye, as in a microscope, is derived from its enabling the eye to approach more nearly to its object than would otherwise be compatible with distinct vision. The common occurrence of walking across the street to read a bill is in fact magnifying the bill by approach; and the observer, at every step he takes, makes a change in the optical arrangement of his eye, to adapt it to the lessening distance between himself and the object of his inquiry. This power of spontaneous adjustment is so unconsciously exerted, that unless the attention be called to it by circ.u.mstances, we are totally unaware of its exercise.

In the case just mentioned the bill would be read with eyes in a very different state of adjustment from that in which it was discovered on the opposite side of the street, but no conviction of this fact would be impressed upon the mind. If, however, the supposed individual should perceive on some part of the paper a small speck, which he suspects to be a minute insect, and if he should attempt a very close approach of his eye for the purpose of verifying his suspicion, he would presently find that the power of natural adjustment has a limit; for when his eye has arrived within about ten inches, he will discover that a further approach produces only confusion. But if, as he continues to approach, he were to place before his eye a series of properly arranged convex lenses, he would see the object gradually and distinctly increase in apparent size by the mere continuance of the operation of approaching. Yet the gla.s.ses applied to the eye during the approach from ten inches to one inch, would have done nothing more than had been previously done by the eye itself during the approach from fifty feet to one foot. In both cases the magnifying is effected really by the approach, the lenses merely rendering the latter periods of the approach compatible with distinct vision.

A very striking proof of this statement may be obtained by the following simple and instructive experiment. Take any minute object, a very small insect for instance, held on a pin or gummed to a slip of gla.s.s; then present it to a strong light, and look at it through the finest needle-hole in a blackened card placed about an inch before it.

The insect will appear quite distinct, and about ten times larger than its usual size. Then suddenly withdraw the card without disturbing the object, which will instantly become indistinct and nearly invisible.

The reason is, that the naked eye cannot see at so small a distance as one inch. But the card with the hole having enabled the eye to approach within an inch, and to see distinctly at that distance, is thus proved to be as decidedly a magnifying instrument as any lens or combination of lenses.

This description of magnifying power does not apply to such instruments as the solar or gas microscope, by which we look not at the object itself, but at its shadow or picture on the wall; and the description will require some modification in treating of the compound microscope, where, as in the telescope, an image or picture is formed by one lens, that image or picture being viewed as an original object by another lens.

It is nevertheless so important to obtain a clear notion of the real nature of the effect produced by a lens applied to the eye, that we will adduce the instance of spectacles to render the point more familiar. If the person who has been supposed to cross the street for the purpose of reading a bill had been aged, the limit to the power of adjustment would have been discovered at a greater distance, and without so severe a test as the supposed insect. The eyes of the very aged generally lose the power of adjustment at a distance of thirty or forty inches instead of ten, and the spectacles worn in consequence are as much magnifying gla.s.ses to them as the lenses employed by younger eyes to examine the most minute objects. Spectacles are magnifying gla.s.ses to the aged because they enable such persons to see as closely to their objects as the young, and therefore to see the objects larger than they could themselves otherwise see them, but not larger than they are seen by the una.s.sisted younger eye.

In saying that an object appears larger at one time, or to one person, than another, it is necessary to guard against misconception. By the apparent size of an object we mean the angle it subtends at the eye, or the angle formed by two lines drawn from the centre of the eye to the extremities of the object. In Fig. 1, the lines A E and B E drawn from the arrow to the eye form the angle A E B, which, when the angle is small, is nearly twice as great as the angle C E D, formed by lines drawn from a similar arrow at twice the distance. The arrow A B will therefore appear nearly twice as long as C D, being seen under twice the angle, and in the same proportion for any greater or lesser difference in distance. The angle in question is called the angle of vision, or the visual angle.

[Ill.u.s.tration: Fig. 1.]

The angle of vision must, however, not be confounded with the angle of the pencil of light by which an object is seen, and which is explained in Fig. 2. Here we have drawn two arrows placed in relation to the eye as before, and from the centre of each have drawn lines exhibiting the quant.i.ty of light which each point will send into the eye at the respective distances.

[Ill.u.s.tration: Fig. 2.]

Now if E F represent the diameter of the pupil, the angle E A F shows the size of the cone or pencil of light which enters the eye from the point A, and in like manner the angle E B F is that of the pencil emanating from B, and entering the eye. Then, since E A F is double E B F, it is evident that A is seen by four times the quant.i.ty of light which could be received from an equally illuminated point at B; so that the nearer body would appear brighter if it did not appear larger; but as its apparent area is increased four times as well as its light, no difference in this respect is discovered. But if we could find means to send into the eye a larger pencil of light, as for instance that shown by the lines G A H, without increasing the apparent size in the same proportion, it is evident that we should obtain a benefit totally distinct from that of increased magnitude, and one which is in some cases of even more importance than size in developing the structure of what we wish to examine. This, it will be hereafter shown, is sometimes done; for the present, we wish merely to explain clearly the distinction between apparent magnitude, or the angle under which the object is seen, and apparent brightness, or the angle of the pencil of light by which each of its points is seen, and with these explanations we shall continue to employ the common expressions magnifying gla.s.s and magnifying power.

[Ill.u.s.tration: Fig. 3.]

The magnifying power of a single lens depends upon its focal length, the object being in fact placed nearly in its princ.i.p.al focus, or so that the light which diverges from each point may, after refraction by the lens, proceed in parallel lines to the eye, or as nearly so as is requisite for distinct vision. In Fig. 3, A B is a double convex lens, near which is a small arrow to represent the object under examination, and the cones drawn from its extremities are portions of the rays of light diverging from those points and falling upon the lens. These rays, if suffered to fall at once upon the pupil, would be too divergent to permit their being brought to a focus upon the retina by the optical arrangements of the eye. But being first pa.s.sed through the lens, they are bent into nearly parallel lines, or into lines diverging from some points within the limits of distinct vision, as from C and D. Thus altered, the eye receives them precisely as if they emanated from a larger arrow placed at C D, which we may suppose to be ten inches from the eye, and then the difference between the real and the imaginary arrow is called the magnifying power of the lens in question.

From what has been said it will be evident that two persons whose eyes differed as to the distance at which they obtained distinct vision, would give different results as to the magnifying power of a lens. To one who can see distinctly with the naked eye at a distance of five inches, the magnifying power would seem and would indeed be only half what we have a.s.sumed. Such instances are, however, rare; the focal length of the eye usually ranges from six to twelve or fourteen inches, so that the distance we first a.s.sumed of ten inches is very near the true average, and is a convenient number, inasmuch as a cipher added to the denominator of the fraction which expresses the focal length of a lens gives its magnifying power. Thus a lens whose focal length is one-sixteenth of an inch is said to magnify 160 times.

When the focal length of a lens is very small, it is difficult to measure accurately the distance between its centre and its object. In such cases the best way to obtain the focal length for parallel or nearly parallel rays is to view the image of some distant object formed by the lens in question through another lens of one inch solar focal length, keeping both eyes open and comparing the image presented through the two lenses with that of the naked eye. The proportion between the two images so seen will be the focal length required. Thus if the image seen by the naked eye is ten times as large as that shown by the lenses, the focal length of the lens in question is one-tenth of an inch. The panes of gla.s.s in a window, or courses of bricks in a wall, are convenient objects for this purpose.

In whichever way the focal length of the lens is ascertained, the rules given for deducing its magnifying power are not rigorously correct, though they are sufficiently so for all practical purposes, particularly as the whole rests on an a.s.sumption in regard to the focal length of the eye, and as it does not in any way affect the actual measurement of the object. To calculate with great precision the magnifying power of a lens with a given focal length of eye, it is necessary that the thickness of the lens be taken into the account, and also the focal length of the eye itself.

We have hitherto considered a magnifying lens only in reference to its enlargement of the object, or the increase of the angle under which the object is seen. A further and equally important consideration is that of the number of rays or quant.i.ty of light by which every point of the object is rendered visible. The naked eye, as shown in Fig. 2, admits from each point of every visible object a cone of light having the diameter of the pupil for its base, and most persons are familiar with that beautiful provision by which in cases of excessive brilliancy the pupil spontaneously contracts to reduce the cone of admitted light within bearable limits. This effect is still further produced in the experiment already described, of looking at an object through a needle-hole in a card, which is equivalent to reducing the pupil to the size of a needle-hole. Seen in this way the object becomes comparatively dark or obscure; because each point is seen by means of a very small cone of light, and a little consideration will suffice to explain the different effects produced by the needle-hole and the lens. Both change the angular value of the cone of light presented to the eye, but the lens changes the angle by bending the extreme rays within the limits suited to distinct vision, while the needle-hole effects the same purpose by cutting off the rays which exceed those limits.

It has been shown that removing a brilliant object to a greater distance will reduce the quant.i.ty of light which each point sends into the eye, as effectually as viewing it through a needle-hole; and magnifying an object by a lens has been shown to be the same thing in some respects as removing it to a greater distance. We have to see the magnified picture by the light emanating from the small object, and it becomes a matter of difficulty to obtain from each point a sufficient quant.i.ty of light to bear the diffusion of a great magnifying power.

We want to perform an operation just the reverse of applying the card with the needle-hole to the eye--we want in some cases to bring into the eye the largest possible pencil of light from each point of the object.

Referring to Fig. 3, it will be observed that if the eye could see the small arrow at the distance there shown without the intervention of the lens, only a very small portion of the cones of light drawn from its extremities would enter the pupil; whereas we have supposed that after being bent by the lens the whole of this light enters the eye as part of the cones of smaller angle whose summits are at C and D. These cones will further explain the difference between large and small pencils of light; those from the small arrow are large pencils; the dotted cones from the large arrow are small pencils.

In a.s.suming that the whole of this light could have been suffered to enter the eye through the lens A B, we did so for the sake of not perplexing the reader with too many considerations at once. He must now learn that so large a pencil of light pa.s.sing through a single lens would be so distorted by the spherical figure of the lens, and by the chromatic dispersion of the gla.s.s, as to produce a very confused and imperfect image. This confusion may be greatly diminished by reducing the pencil; for instance, by applying a stop, as it is called, to the lens, which is neither more nor less than the needle-hole applied to the eye. A small pencil of light may be thus transmitted through a single lens without suffering from spherical aberration or chromatic dispersion any amount of distortion which will materially affect the figure of the object; but this quant.i.ty of light is insufficient to bear diffusion over the magnified picture, which is therefore too obscure to exhibit what we most desire to see--those beautiful and delicate markings by which one kind of organic matter is distinguished from another. With a small aperture these markings are not seen at all: with a large aperture and a single lens they exhibit a faint nebulous appearance enveloped in a chromatic mist, a state which is of course utterly valueless to the naturalist, and not even amusing to the amateur.

It becomes therefore a most important problem to reconcile a large aperture with distinctness, or, as it is called, _definition_; and this has been done in a considerable degree by effecting the required amount of refraction through two or more lenses instead of one, thus reducing the angles of incidence and refraction, and producing other effects which will be shortly noticed. This was first accomplished in a satisfactory manner by--

DR. WOLLASTON'S DOUBLET,

invented by the celebrated philosopher whose name it bears; it consists of two plano-convex lenses (Fig. 4) having their focal lengths in the proportion of 1 to 3, or nearly so, and placed at a distance which can be ascertained best by actual experiment. Their plane sides are placed towards the object, and the lens of shortest focal length next the object.

[Ill.u.s.tration: Fig. 4.]

It appears that Dr. Wollaston was led to this invention by considering that the Achromatic Huyghenean Eye-piece, which will be hereafter described, would, if reversed, possess similar good properties as a simple microscope. But it will be evident when the eye-piece is understood, that the circ.u.mstances which render it achromatic are very imperfectly applicable to the simple microscope, and that the doublet, without a nice adjustment of the stop, would be valueless. Dr.

Wollaston makes no allusion to a stop, nor is it certain that he contemplated its introduction, although his illness, which terminated fatally soon after the presentation of his paper, may account for the omission.

The nature of the corrections which take place in the doublet is explained in the annexed diagram (Fig. 5), where L O L' is the object, P a portion of the pupil, and D D the stop, or limiting aperture.

Now, it will be observed that each of the pencils of light from the extremities L L' of the object is rendered eccentrical by the stop, and of consequence each pa.s.ses through the two lenses on opposite sides of their common axis O P; thus each becomes affected by opposite errors, which to some extent balance and correct each other. To take the pencil L, for instance, which enters the eye at R B, R B; it is bent to the right at the first lens, and to the left at the second; and as each bending alters the direction of the blue rays more than the red, and, moreover, as the blue rays fall nearer the margin of the second lens, where the refraction, being more powerful than near the centre, compensates in some degree for the greater focal length of the second lens, the blue and red rays will emerge very nearly parallel, and of consequence colorless to the eye. At the same time the spherical aberration has been diminished by the circ.u.mstance that the side of the pencil which pa.s.ses one lens nearest the axis pa.s.ses the other nearest the margin.

This explanation applies only to the pencils near the extremities of the object. The central pencil, it is obvious, would pa.s.s both lenses symmetrically; the same portions of light occupying nearly the same relative places on both lenses. The blue light would enter the second lens nearer to its axis than the red, and being thus less refracted than the red by the second lens, a small amount of compensation would take place, quite different in principle and inferior in degree to that which is produced in the eccentrical pencils. In the intermediate s.p.a.ces the corrections are still more imperfect and uncertain; and this explains the cause of the aberrations which must of necessity exist even in the best-made doublet. It is, however, infinitely superior to a single lens, and will transmit a pencil of an angle of from 35 to 50 without any very sensible errors. It exhibits, therefore, many of the usual test-objects in a very beautiful manner.

[Ill.u.s.tration: Fig. 5.]

[Ill.u.s.tration: Fig. 6.]

The next step in the improvement of the simple microscope bears more a.n.a.logy to the eye-piece. This improvement was made by Mr. Holland, and it consists (as shown in Fig. 6) in subst.i.tuting two lenses for the first in the doublet, and retaining the stop between them and the third. The first bending, being thus effected by two lenses instead of one, is accompanied by smaller aberrations, which are therefore more completely balanced or corrected at the second bending, in the opposite direction, by the third lens. This combination, though called a triplet is essentially a doublet, in which the anterior lens is divided into two. For it must be recollected that the first pair of lenses merely accomplishes what might have been done, though with less precision, by one; but the two lenses of the doublet are opposed to each other; the second diminis.h.i.+ng the magnifying power of the first.

The first pair of lenses in the triplet concur in producing a certain amount of magnifying power, which is diminished in quant.i.ty and corrected as to aberration at the third lens by the change in relation to the position of the axis which takes place in the pencil between what is virtually the first and second lens. In this combination the errors are still further reduced by the close approximation to the object which causes the refractions to take place near the axis. Thus the transmission of a still larger angular pencil, namely 65, is rendered compatible with distinctness, and a more intense image is presented to the eye.

Every increase in the number of lenses is attended with one drawback, from the circ.u.mstance that a certain portion of light is lost by reflection and absorption each time that the ray enters a new medium.

This loss bears no sensible proportion to the gain arising from the increased aperture, which, being as the square of the diameter, multiplies rapidly; or, if we estimate by the angle of the admitted pencil, which is more easily ascertained, the intensity will be as the square of twice the tangent of half the angle. To explain this, let D B (Fig. 7) represent the diameter of the lens, or of that part of it which is really employed; C A the perpendicular drawn from its centre, and A B, A D, the extreme rays of the incident pencil of light DAB. Then the diameter being 2 C B, the area to which the intensity of vision is proportional will be (2 C B), and C B is evidently the tangent of the angle C A B, which is half the angle of the admitted pencil D A B. Or, if _a_ be used to denote the angular aperture, the expression for the intensity is (2 tan. _a_) which increases so rapidly with the increase of _a_ as to make the loss of light by reflection and absorption of little consequence.

[Ill.u.s.tration: Fig. 7.]

The combination of three lenses approaches, as has been stated, very close to the object; so close, indeed, as to prevent the use of more than three; and this const.i.tutes a limit to the improvement of the simple microscope, for it is called a simple microscope, although consisting of three lenses, and although a compound microscope may be made of only three or even two lenses; but the different arrangement which gives rise to the term compound will be better understood when that instrument is explained.

Before we proceed to describe the simple microscope and its appendages, it will be well to explain such other points in reference to the form and materials of lenses as are most likely to be interesting.

A very useful form of lens was proposed by Dr. Wollaston, and called by him the Periscopic lens. It consisted of two hemispherical lenses, cemented together by their plane faces, having a stop between them to limit the aperture. A similar proposal was made Mr. Coddington, who, however, executed the project in a better manner, by cutting a groove in a whole sphere, and filling the groove with opaque matter. His lens, which is the well-known Coddington lens, is shown in Fig. 8. It gives a large field of view, which is equally good in all directions, as it is evident that the pencils A A and B B pa.s.s through under precisely the same circ.u.mstances. Its spherical form has the further advantage of rendering the position in which it is held of comparatively little consequence. It is therefore very convenient as a hand-lens, but its definition is of course not so good as that of a well-made doublet or achromatic lens.

[Ill.u.s.tration: Fig. 8.]

Another very useful form of doublet was proposed by Sir John Herschel, chiefly like the Coddington lens, for the sake of a wide field, and chiefly to be used in the hand. It is shown in Fig. 9; it consists of a double convex or crossed lens, having the radii of curvature as 1 to 6, and of a plane concave lens whose focal length is to that of the convex lens as 13 to 5.

Various, indeed innumerable, other forms and combinations of lenses have been projected, some displaying much ingenuity, but few of any practical use. In the Catadioptric lenses the light emerges at right angles from its entering direction, being reflected from a surface cut at an angle of 45 degrees to the axes of the curved surfaces.

[Ill.u.s.tration: Fig. 9.]

It was at one time hoped, as the precious stones are more refractive than gla.s.s, and as the increased refractive power is unaccompanied by a correspondent increase in chromatic dispersion, that they would furnish valuable materials for lenses, inasmuch as the refractions would be accomplished by shallower curves, and consequently with diminished spherical aberration. But these hopes were disappointed; everything that ingenuity and perseverance could accomplish was tried by Mr. Varley and Mr. Pritchard, under the patronage of Dr. Goring. It appeared, however, that the great reflective power, the doubly-refracting property, the color, and the heterogeneous structure of the jewels which were tried, much more than counterbalanced the benefits arising from their greater refractive power, and left no doubt of the superiority of skillfully made gla.s.s doublets and triplets. The idea is now, in fact, abandoned; and the same remark is applicable to the attempts at constructing fluid lenses, and to the projects for giving to gla.s.s other than spherical surfaces--none of which have come into extensive use.